|
KMID : 1100720230430020174
|
|
Annals of Laboratory Medicine
2023 Volume.43 No. 2 p.174 ~ p.179
|
|
|
Performance Comparison Between Fourier-Transform Infrared Spectroscopy?based IR Biotyper and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Strain Diversity
|
|
Jun Son-Young
Kim Young-Ah
Lee Suk-Jun
Jung Woon-Won
Kim Hyun-Sook
Kim Sung-Soo
Kim Hyun-Soo
Yong Dong-Eun
Lee Kyung-Won
|
|
|
Abstract
|
|
|
Background: Development of an accessible method to routinely evaluate the clonality of strains is needed in microbiology laboratories. We compared the discriminatory power of the Fourier-transform infrared (FTIR) spectroscopy?based IR Biotyper (Bruker Daltonics GmbH, Bremen, Germany) to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), using whole-genome sequencing (WGS) as the reference method.
Methods: Eighty-three extended-spectrum β-lactamase?producing Escherichia coli isolates were tested using WGS, MALDI-TOF MS, and IR Biotyper. Simpson’s diversity index (SDI), a statistical analysis for testing the homogeneity of a dendrogram, and the adjusted Rand index (aRI) were used to compare the discriminatory ability between typing tests.
Results: The SDI (95% confidence interval) was 0.969 (0.952?0.985) for WGS, 0.865 (0.807?0.924) for MALDI-TOF MS, and 0.974 (0.965?0.983) for IR Biotyper. Compared with WGS, IR Biotyper showed compatible diversity, whereas MALDI-TOF MS did not. The concordance and aRI improved from 66.3% to 84.3% and from 0.173 to 0.538, respectively, for IR Biotyper versus MALDI-TOF MS with WGS as the reference method. IR Biotyper showed substantially improved performance in strain typing compared with MALDI-TOF MS.
Conclusions: IR Biotyper is useful for diversity analysis with improved discriminatory power over MALDI-TOF MS in comparison with WGS as a reference method. IR Biotyper is an accessible method to evaluate the clonality of strains and could be applied in epidemiological analysis during an outbreak of a health care facility, as well as for research on the transmission of resistant bacteria in community settings.
|
|
|
KEYWORD
|
|
|
Fourier-transform infrared, Matrix-assisted laser desorption-ionization, Spectrometry, Whole-genome sequencing
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
   
|
|